SMILEs
STRUCTURAL MOLECULAR IMAGING LIGHT ENHANCED SPECTROSCOPIES

People

Tania Limongi

Research Scientists

Introduction

Her research interest is the development of new tissue engineering approaches and of novel devices and methods for ultrasensitive single molecule detection, imaging and spectroscopy, both in in vivo and invitro cells experiment. Designing versatile materials that can be used for the optimization of biological sample preparation is highly desired yet still challenging. Her main activity in KAUST regards the use of superhydrophobic surface as an effective solution offering the possibility to perform different high-resolution imaging measurements. It consists in simple but effective system allowing fast and reproducible specimen preparation for the obtainment of isolated plasma membranes images. By means of micro-nano featured surfaces and sample deposition in super hydrophobicity condition, she was able to carry out high resolution imaging to overcome the bottlenecks in the membrane protein research, for the study of cellular membrane and integral membrane proteins that play critical roles also in the management of various pathological conditions of diseases. By simultaneously recording the topography and chemical fingerprint of the protein-tethered lipid bilayer, it will be possible to probe locally the chemical signature of membranes and to provide a local map of its surface morphology.

Selected Publications

Coluccio M.L., Gentile F., Das G, Nicastri A., Perri A.M., Candeloro P., Perozziello G., Proietti R.Z., Totero Gongora J.S., Alrasheed S., Fratalocchi A., Limongi T., Cuda G., Di Fabrizio E. Detection of single amino acid mutation in human breast cancer by disordered plasmonic self-similar chain (2015) Science Advances, 1(8), e1500487. DOI: 10.1126/sciadv.1500487.

Marini M., Falqui A., Moretti M., Limongi T., Allione M., Genovese A., Lopatin S., Tirinato L., Das G., Torre B., Giugni A., Gentile F., Candeloro P. and Di FabrizioE. The structure of DNA by direct imaging (2015) Science Advances ,1, (7), e1500734. DOI: 10.1126/sciadv.1500734.

Limongi T., Miele E., Shalabaeva V., La Rocca R., Schipani R., Malara N., De Angelis F., Giugni A., di Fabrizio E. Development, characterization and cell cultural response of a 3D biocompatible micro-patterned Poly--caprolactone scaffolds designed and fabricated integrating lithography and micromolding fabrication techniques (2015) Journal of Tissue Science and Engineering, 6(1) doi: 10.4172/2157-7552.1000145.

Malara N.M.,  Trunzo V., Musolino G., Aprigliano S., Rotta G., Macrina L., Limongi T., Gratteri S.,  Di Fabrizio E., Renzulli A., Fini M., Mollace V. Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application (2015)  IJC Heart & Vasculature,  6(1) pp. 48–53.

Malara N., Coluccio M.L., Limongi T., Asande M., Trunzo V., Cojoc G., Raso C., Candeloro P., Perozziello G., Raimondo R., De Vitis S., Roveda L., Renne M., Prati U., Mollace V., Di Fabrizio E. Folic Acid Functionalized Surface Highlights 5-Methylcytosine-Genomic Content within Circulating Tumor Cells (2014) Small, 10(21) pp. 4324-31. doi: 10.1002/smll.201400498.

Education

•    Ph.D. on Microsystems engineering, 2004, Tor Vergata University, Rome, Italy.

•    Degree on Biology (Laurea), 1999, L'Aquila University, Italy.

•    Scientifical High School Diploma, 1992, Liceo Scientifico “Galileo Galilei” (Pescara). Mark: 50/60