SMILEs
STRUCTURAL MOLECULAR IMAGING LIGHT ENHANCED SPECTROSCOPIES

People

Manola Moretti

Postdoctoral Fellows

Introduction

Since her Master Thesis, Manola has been working at the boundary of various disciplines which include, broadly speaking, biology, chemistry and physics. Going deeper into that, she has dedicated her working activity to the development of tools for single molecule detection applied to molecular biology and medicine by means of nanotechnology. Recently she was devoted to the exploitment of surface plasmon resonance effect on nanofabricated AFM tips to characterize membrane proteins, which are known to be dramatically important in the onset of several diseases and unfortunately very difficult to characterize. Her scope is now to set up a new characterization tool able to take a chemical picture of a very well-known protein (titin protein) while it is mechanically unfolded. On the other hand, the very ambitious goal is to achieve the same task on the living cell surface membrane. AFM force spectroscopy will be the elected technique and, together with nanofabrication (nanostructured plasmonic waveguides for light confinement), molecular biology (protein engineering) and chemical functionalization (chemical attachment of active groups to proteins and substrates) she will attain this objective.

Selected Publications

M Moretti, G Das, B Torre, M Allione, E Di Fabrizio. Tip Enhanced Raman Spectroscopy of Rhodamine 6G on nanostructured gold substrate. Optics and Lasers in Engineering 2015; in press.

R Intartaglia, S Beke, M Moretti, F de Angelis, A Diaspro. Fast and cost-effective fabrication of large-area plasmonic transparent biosensor array. Lab on a Chip 01/2015; 15(5). DOI:10.1039/C4LC01293C.

 A Giugni, M Allione, B Torre, G Das, M Francardi, M Moretti, M Malerba, G Perozziello, P Candeloro, E Di Fabrizio. Adiabatic nanofocusing: spectroscopy, transport and imaging investigation of the nano world. Journal of optics 11/2014; 16(11):114003. DOI:10.1088/2040-8978/16/11/114003.

T Limongi, L Ferrara, G Das, M Moretti, M Marini, E Miele, A Accardo, R Raimondo, F Gentile, E Di Fabrizio. Superhydrophobic Devices Molecular Detection. Novel Approaches for Single Molecule Activation and Detection, Edited by Fabio Benfenati, Enzo Di Fabrizio, Vincent Torre, 01/2014: chapter 4: pages 15; Springer Berlin Heidelberg.

C Marasini, E Jacchetti, M Moretti, C Canale, O Moran, M Vassalli. Visualization of single proteins from stripped native cell membranes: A protocol for high-resolution atomic force microscopy. Microscopy Research and Technique 07/2013; 76(7). DOI:10.1002/jemt.22223

Education

2003-2008 PhD TASC-INFM, LILith Lab, Trieste, Italy

2002 Laurea Degree in Plant Biotechnology, Udine University, Italy