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STRUCTURAL MOLECULAR IMAGING LIGHT ENHANCED SPECTROSCOPIES

Localization of single strand DNA and Rad51 repair protein by means of SERS technique on superhydrophobic devices

Localization of single strand DNA and Rad51 repair protein by means of SERS technique

Details

In this work, single strand DNA and DNA incubated with Rad51 protein (nucleoproteins) have been suspended on superhydrophobic surfaces. These fibers were investigated with techniques such as electron microscopy and Raman spectroscopy to illustrate the variations in the Raman spectra. The use of Enhanced Raman Spectroscopy revealed that the method developed serves to investigate directly the nucleoprotein formation and the Rad51 protein distribution. The data were further confirmed by direct imaging with Scanning and Transmission Electron Microscopy. This combined approach represents a methodological improvement for the understanding of the interactions occurring between proteins and DNA and, more in general, biological molecules.‚Äč